DNA probes for detection of copper resistance genes in Xanthomonas campestris pv. vesicatoria

Garde, S.; Bender, C.L.

DNA probes for detection of copper resistance genes in Xanthomonas campestris pv. vesicatoria

1991

Garde, S. | Bender, C.L.

The copper resistance (Cur) genes encoded on pXV10A, a 190-kb plasmid in Xanthomonas campestris pv. vesicatoria XV10, were isolated on a 44-kb cosmid clone designated pCuR1. Tn5 mutagenesis of pCuR1 indicated that a 4.0-kb region was required for copper resistance. Three restriction fragments located within the 4.0-kb region demonstrated high specificity for the Cur genes present in X. campestris pv. vesicatoria and will be useful in monitoring the presence of these genes in the environment.

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AGROVOC Keywords

cloning copper copper dna probes drug resistance genes genes genetics pharmacology plasmids plasmids strains xanthomonas campestris

Bibliographic information

Published in

Applied and environmental microbiology

Volume 57 Issue 8 Pagination 2435 - 2439 ISSN 0099-2240

Other Subjects

Restriction mapping; Drug effects; Southern; Blotting; Resistance; Microbial; Bacterial; Molecular

Language

English

Type

Journal Article; Text

In AGRIS since: 2024-02-28

Format: MODS

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DNA probes for detection of copper resistance genes in Xanthomonas campestris pv. vesicatoria

1991

AGROVOC Keywords

Bibliographic information