Effect of sucrose on in vitro germplasm preservation in Dendrobium friedericksianum Rchb.f
2019
Sukhumpinij, P.
The present study was conducted to examine the effect of sucrose on the reduction of in vitro preservation of Dendrobium friedericksianum Rchb.f. seedlings after prolonged subculture in Vacin and Went (1949) (VW) medium supplemented with different concentrations of sucrose. D. friedericksianum is a native Thai wild orchid found in the eastern provinces of Thailand, especially in Chanthaburi Province. Currently, it is a highly endangered species because of illegal forest collection, decreasing rate of natural breeding, and environmental changes leading to a higher risk of extinction. The aim of the present study was to develop an effective protocol for in vitro storage of this species. Plantlets of D. friedericksianum were cultured on VW media supplemented with 0, 2, 4, 6, 8, and 10% sucrose. Plant height decreased when 8 and 10% sucrose was applied (decrease of 2.25 and 1.97 cm, respectively), which was significantly higher than the height decrease for those provided with 0, 2, 4, and 6% sucrose (decrease of 2.85, 4.75, 3.27, and 2.75 cm, respectively). Plants treated with 10% sucrose could be preserved for 6 months without subculture and plant height was decreased by only 2.78 cm. The pseudo-bulb diameter when treated with 10% sucrose was 0.08 cm higher than that treated with the basal medium (2% sucrose).
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