Structure and in vitro growth of zygotic embryos of taro (Colocasia esculenta var. antiquorum)
1986
Nyman, L.P. | Webb, E.L. | Gu, Z. | Arditti, J.
Zygotic embryos of taro, Colocasia esculenta var. antiquorum were examined using both light and scanning electron microscopy and cultured on Linsmaier-Skoog (LS) medium without the addition of growth regulators. Embryos present within mature seed consist of a hypocotyl-root axis and an undeveloped cotyledon and are surrounded by two major types of endosperm cells, aleurone and starchy endosperm. Embryos cultured on LS medium developed into mature plants only in the presence of endosperm tissue. Excised embryos turned green after 2–4 d in culture and reached a rapid growth period between days 4 and 6. Culture of taro embryos leading to viable plantlet development depends upon (1) removal of the outer and inner integument, and (2) the presence of endosperm tissue (including an intact aleurone layer).
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