Effects of dietary n‐6 polyunsaturated fatty acids on growth performance, body composition, haematological parameters and hepatic physiology of juvenile tiger puffer (Takifugu rubripes)
2019
Xu, Houguo | Liao, Zhangbin | Zhang, Qinggong | Wei, Yuliang | Liang, Mengqing
A 74‐day feeding trial was conducted to investigate the efficacy of n‐6 polyunsaturated fatty acid (n‐6 PUFA) in diets for tiger puffer. Six experimental diets with graded levels of n‐6 PUFA (from 6.01 to 37.98 g/kg) replacing saturated fatty acids (SFAs) were used in this study. Increasing levels of n‐6 PUFA resulted in decreased weight gain (from 472.6% to 349.4%), feed efficiency and crude protein content in fish, but increased lipid accumulation in whole fish and liver. Liver samples from fish fed the lowest and highest level of n‐6 PUFA were subjected to transcriptomic assay. Analysis of the differentially expressed genes showed that high dietary levels of n‐6 PUFA tended to induce glycolysis and triacylglycerol biosynthesis, but reduce synthesis of amino acid and protein, and cause impairment to liver health. The mitochondrial DNA copy number, activity of mitochondrial respiratory chain complex I and glycogen content in the liver, which indicate energy supply status, significantly decreased with increasing levels of dietary n‐6 PUFA. In conclusion, high n‐6 PUFA/SFA ratios in diet led to reduced energy supply (by approximately 50%) and increased hepatic lipid accumulation (by 55%), which probably consequently negatively affect protein synthesis, liver health and growth of tiger puffer.
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