In the years 2003–2012 the effects of nitrogen fertilization and term of harvest on the dry matter yield and biomass quality of Miscanthus × giganteus were examined. The harvest was carried out each year in the autumn and in the spring following year. No significant differences in yields between the sites were observed but the effect of weather conditions in individual years dominated. The nitrogen fertilization increased average biomass yields at the site Prague-Ruzyně by about 14% at the dose of 100 kg/ha and at the site Lukavec by about 11% at the dose of 150 kg/ha in comparison without N fertilization. Average yields of dry matter at Prague-Ruzyně 19.60 t/ha and at Lukavec 18.24 t/ha were achieved at the autumn term of harvest. The loss of biomass over the winter period was 24.3% at Prague-Ruzyně and 24.0% at Lukavec. In the spring term of harvest lower contents of all monitored elements were detected in the biomass of Miscanthus compared to the autumn term of harvest.
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