De novo assembly and annotation of the marine mysid (Neomysis awatschensis) transcriptome
2016
Kim, Hŭi-su | Hwang, Dae-Sik | Lee, Bo-Young | Park, Jun Chul | Lee, Young-Hwan | Lee, Jae-seong
We sequenced the whole transcriptome of the marine mysid Neomysis awatschensis (Crustacea: Mysidacea) using Illumina RNA-seq. De novo assembly was performed with 14,018,702 raw reads using Trinity, resulting in 82,434 contigs. Transdecoder found 22,141 candidate coding contigs with homology to other species by BLAST analysis. Functional gene annotation was performed by Gene Ontology, InterProScan, and KEGG pathway analyses. We generated an expressed gene catalog for the mysid N. awatschensis to serve as a resource for marine environmental genomic and ecotoxicogenomic studies focused on uncovering the molecular mechanisms underlying the responses of N. awatschensis to environmental stressors and chemicals.
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