Matrix metalloproteinase-9 activity in ewes' milk and its relationship to somatic cell counts
2022
Ianni, Andrea | Bennato, Francesca | Martino, Camillo | Saletti, Maria Antonietta | Pomilio, Francesco | Martino, Giuseppe
The presence and the activity of gelatinase-B/matrix metalloproteinase 9 (MMP-9) in ewes' milk was evaluated with a particular focus on the possible correlation with the somatic cell count (SCC). Forty five individual milk samples obtained from lacaune sheep, previously analysed for SCC with values ranging from 5.0 × 10⁴ to 1.6 × 10⁷ cells mL⁻¹, gave overall gelatinolytic activity between 0.53 ± 0.05 and 4.53 ± 0.55 fluorescence units min⁻¹ mg⁻¹ protein. Zymography showed that the variation in the gelatinolytic activity depended on variations in the specific activity of MMP-9; no significant differences in MMP-2 (gelatinase-A) activity were observed. Pearson's correlation calculations gave a fair correlation between the presence of somatic cells and the enzymatic activity. MMP-9 in the context of proteolytic activities related to the presence of somatic cells acquires greater importance taking into account the hydrolytic potential of MMP-9 versus casein, evaluated in this study by exploiting a molecular docking approach.
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