The impact of melaphene on the expression of chloroplastic chaperone protein HSP70B and photosynthetic pigments in cells of Chlamydomonas reinhardtii
2009
Ermokhina, O. V. | Belkina, G. G. | Oleskina, Yu. P. | Fattakhov, S. G. | Yurina, N. P.
The effects of growth regulator of the new generation--melamine salt of bis(oxymethyl)phosphine acid (melaphene)--on culture growth, pigment and protein content, and the induction of protective chloroplastic chaperone HSP70B in Chlamydomonas reinhardtii CW15 cells were studied. Melaphene exhibited 10-30% growth inhibition at 10⁻⁹-10⁻²% concentration. At 10⁻⁹-10⁻⁴% of melaphene electrophoretic concentration, the pattern of cellular proteins was similar to the control. The alterations in protein content of algae cells were detected only at 10⁻²% concentration. The content of chlorophyll and carotenoids in melaphene-treated cells was 17-40% lower than in the control. Melaphene at 10⁻⁹-10⁻²% concentration inhibited HSP70B induction by 39-43% compared to untreated cells. The potential mechanism of melaphene effect might involve its influence on nuclear gene expression.
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