Extraction of soil phosphorus with calcium chloride solution for prediction of plant availability
1995
Hylander, L.D. | Svensson, H.I. | Siman, G.
Soil and vegetative samples of ley and cereals were collected four times during the growing season from field and pot trials with different phosphorus (P) fertilisation levels. The soil samples, dried and of field moisture condition, respectively, were extracted by 0.01M calcium chloride (CaCl2) at two different soil:extractant ratios (1:2 and 1:10), and analysed by inductively coupled plasma emission spectrometry (ICP) for content of P. The plant samples were digested in concentrated nitric acid (HNO3) and the P content determined by ICP. Calcium chloride-extractable P content was lowest in the middle of the growing season, while plant P was highest in the beginning of the season. Phosphorus extracted by CaCl2 solution was higher at a soil:extractant ratio of 1:10 than at 1:2, and also when drying the soil before extraction. A soil:extractant ratio of 1:2 minimizes the risk of coming too near the limit of determination. However, if organic soils are also to be included, a ratio of 1:10 has to be used in order not to have all the solution absorbed by some types of soils. The solution of ammonium lactate/acetic acid (AL) extracted nearly two powers of ten more P than CaCl2 solution. There was a good relationship between the methods. If calcareous or very acid soils had been included, a less good relation would have been expected. Plant P content varied more in straw than in grain between different treatments. Measuring CaCl2-extractable P with ICP might be able to predict plant uptake of P by plants. This would be a great advantage when using 0.01M CaCl2 as a universal extractant.
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