Qualitative and Quantitative Phytochemical Analysis and In-vitro Antioxidant Activity of Caesalpinia bonduc (L.) Seeds
2015
Kumar Sagar, Manoj | Upadhyaya, Kumud | Rishishwar, Poonam
Caesalpinia bonduc prickly shrub found throughout the hotter regions of India, Myanmar and Sri Lanka. The individual fractions were subjected to qualitative chemical investigation for the identification of the type of active principles. Hexane and ethanolic extract showed the presence of carbohydrate, saponin, phytosterol, protein, amino acid, tannin, phenolic compounds and flavonoids. It was found that all extracts contain major chemical constituents for various pharmacological activities. The present study deals with the standardization of the plant seed on the basis of various phytochemical and physicochemical parameters. The extract enhanced free radical scavenging activity of stable radical 2,2-diphenyl-1-picryl-hydrazyl (DPPH), nitric oxide and hydroxyl redical in In-vitro assay methods. The result of the study indicate that the methanolic extract of C. bonduc possess significant antioxidant activity. The observed pharmacological activity may be due to the presence of phytochemicals like flavonoid, alkaloids and tannins present in the plant extract with various biological activities.
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