A Carotenoid Cleavage Dioxygenase from Vitis vinifera L.: functional characterization and expression during grape berry development in relation to C₁₃-norisoprenoid accumulation
2005
Mathieu, Sandrine | Terrier, Nancy | Procureur, Jérôme | Bigey, Frédéric | Gůnata, Ziya
A potential Carotenoid Cleavage Dioxygenase (CCD) gene was identified among a Vitis vinifera L. EST collection and a full-length cDNA (VvCCD1) was isolated. Recombinant expression of VvCCD1 confirmed that the gene encoded a functional CCD. Experimental evidence was obtained that VvCCD1 cleaves zeaxanthin symmetrically yielding 3-hydroxy-{szligbeta}-ionone, a C₁₃-norisoprenoidic compound, and a C₁₄-dialdehyde. Expression of the gene was studied by real-time PCR at different developmental stages of grape berries from Muscat of Alexandria and Shiraz cultivars. A significant induction of the gene expression approaching véraison was observed in both cultivars. In parallel, the C₁₃-norisoprenoid level increased from véraison to maturity in both cultivars.
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