Impact of bleaching on subcritical water- and Formosolv-pretreated tulip tree to enhance enzyme accessibility
2013
Myint, Aye Aye | Kim, Dae Sung | Lee, Hun Wook | Yoon, Junho | Choi, In-Gyu | Choi, Joon Weon | Lee, Youn-Woo
A novel method was developed for fractionating cellulose microfibrils from forest residue (tulip tree sawdust) to enhance cellulose digestibility, particularly at minimum enzyme loadings. This method involved three main stages: selective hemicellulose solubilization by subcritical water (SCW) pretreatment, delignification of the SCW-pretreated solids using the Formosolv process, and deformylation/bleaching of the cellulose pulp with alkaline hydrogen peroxide solution. This process produced nearly 98% white cellulose microfibrils with 23-fold higher conversion to glucose as compared to the raw substrate after 72h of enzymatic hydrolysis. This study showed that cellulose swelling had the greatest effect on the enzymatic hydrolysis efficiency of delignified pulp obtained by the Formosolv process.
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