Betalain synthesis by hairy root of red beet cultured in vitro under different light quality
2002
Boo, H. | Shin, K. | Heo, J. | Jeong, J. | Paek, K.
The pigment synthesis of many plants has been usually influenced by various factors including light, temperature, nutrient source, enzyme, growth regulator and/or light quality. The present experiment was conducted to investigate the effect of Light-Emitting Diodes (LEDs) on betalain synthesis in hairy root of red beet (Beta vulgaris L.) cultured in vitro. The hairy roots of red beet, which cultured in the 1/4 liquid MS medium for 14 days were used as a plant material. A bioreactor of 1 L in air volume was used as a culture vessel. The hairy roots were cultured in a growth chamber at controlled air temperature of 25°C and relative humidity of 70%. Blue lights (B), Red lights (R), and the mixing lights of B+R (BR), B+Far-red (BFr), and Red+Far-red (RFr) were used as a light source with a photosynthetic photon flux of 50 μmol m-2 s-1. The growth of hairy root under different light conditions showed a dependence of the biomass formation (fresh and dry weight) on light quality. Sugar concentrations were greatest in hairy roots grown under the R+B and B light, and lowest in hairy roots grown under the R and R+F light. The betacyanin concentration per fresh weight of the hairy root on day 14 was 4.2 times greater in BFr treatment than in R treatment, while their concentration was not significantly different with the B, BR, and fluorescent lamp treatments. Betacyanin/betaxanthin ratio was the highest in BFr treatment and was the lowest in R treatment.
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