Characterization of apple cultivars in terms of allergenicity
2012
Paprstein, F. | Proskova, A. | Kminkova, M. | Honzova, S. | Setinova, I. | Kvacova, A.
Growing of different apple cultivars is widespread in the territory of central Europe. Apples are recommended by nutritionists because of their high content in vitamins and dietary fibers. Unfortunately the presence of several allergens in apple pulp and skin causes allergy problems for some consumers. Allergen Mal d1, which causes swelling of lips and oral cavity, is the most important. At present, the cultivar ‘Golden Delicious’ is commercially very successful. Unfortunately a high content of this allergen was detected in this cultivar. Using 17 selected apple cultivars, the allergenicity was studied by prick to prick tests in patients with clinically detected apple allergy. Pressed apple juice was used for basophil activation testing. Intensity of patient’s allergic reaction to particular cultivars was confirmed by SDS-PAGE electrophoresis and immunity reaction of blood serum by Western blot method. Results partly differed depending on the method used. The highest content of allergen was proved for cultivars ‘Resista’, ‘Chodske’, ‘Panenske’ and ‘Rubin’. The lowest content of allergens was found for cultivars ‘Belle de Boskoop’, ‘Florina’ and ‘Topaz’.
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