Creation of blast disease-resistant rice varieties with modern DNA-markers
2015
Dubina, E. V. | Mukhina, Zh. M. | Kharitonov, E. M. | Shilovskiy, V. N. | Kharchenko, E. S. | Esaulova, L. V. | Korkina, N. N. | Maximenko, E. P. | Nikitina, I. B.
Based on modern technologies of molecular DNA-markers, blast disease–resistance genes (Pi-ta, Pi-b, Pi-1, Pi-2, and Pi-33) were introgressed and pyramided into domestic rice varieties to give them longterm disease resistance. For that purpose, this case study uses SSR-markers closely linked to these genes, as well as intragenic markers of genes Pi-ta and Pi-b. Multiplex PCR systems were created for simultaneous identification of two resistance genes in the hybrid progeny for the following combinations: Pi-1 + Pi-2, Pi-ta + Pi-b, Pi-ta + Pi-33.
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