Determination of the total content of some sulfonamides in milk using solid-phase extraction coupled with off-line derivatization and spectrophotometric detection
2015
Dmitrienko, Stanislava G. | Kochuk, Elena V. | Tolmacheva, Veronika V. | Apyari, Vladimir V. | Zolotov, I︠U︡. A. (I︠U︡riĭ Aleksandrovich)
A simple screening method for isolation and determination of the total content of some sulfonamides in milk using solid-phase extraction and a color reaction is described. This procedure is based on SPE of sulfonamides on hypercrosslinked polystyrene, elution with acetonitrile and off-line derivatization with p-dimethylaminocinnamaldehyde in acetonitrile followed by spectrophotometric determination. The reaction produces intense violet–red color and can be easily used both for quantitation of sulfonamides using spectrophotometry and for naked-eye semi-quantitative estimation. Maximum absorption of the reaction product was determined at 540nm. The Lambert–Beer’s law was obeyed in the range of 0.07–3.0μgmL−1 in eluate, with the squared correlation coefficient (R2) of 0.9875–0.9995, and the relative standard deviation (RSD) of 3–4%. The limits of SAs detection using preconcentration were of 0.02–0.03μgmL−1. The proposed method can be recommended as a routine screening method for quantitation of sulfonamides in milk.
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