Physiological basis of UV-C induced resistance to Botrytis cinerea in tomato fruit. V. Constitutive defence enzymes and inducible pathogenesis-related proteins
2009
Charles, Marie Thérèse | Tano, Kablan | Asselin, Alain | Arul, Joseph
Changes in the protein content and profile of postharvest tomato fruit treated with the hormetic dose (3.7kJm⁻²) of ultraviolet light C (UV-C) at the mature green stage was investigated. In UV-C treated fruits, the total protein content increased until 10 d after treatment and decreased thereafter during a 30 d storage period; whereas in control fruit, protein content decreased constantly throughout the storage period. Using polyacrylamide gel electrophoresis (PAGE) it was shown that UV-C treatment affected the protein profile of tomato fruit in several manners: (1) UV-C repressed the expression of some proteins presumably associated with ripening; (2) it enhanced the expression of several constitutive proteins, of which one was an acidic β-1,3-glucanase, three acidic chitinases and three basic chitinases; and (3) it induced the synthesis of at least 5 new proteins of which four were basic proteins. Among the proteins induced by UV-C, three (a basic β-1,3-glucanase and two acidic chitinases) were apparently pathogenesis-related proteins as they were also induced by inoculation with Botrytis cinerea. The molecular mass (MM) of five of the UV-C induced proteins was determined using SDS-PAGE. Their molecular masses were 45, 39.4, 34.6, 10 and 8.9kDa. The UV-C induced β-1,3-glucanase had a MM of 33.1kDa. The MM of two constitutive chitinases were 48.3 and 30.5kDa, and those of the two UV-C and pathogenesis-induced chitinases were 37.1 and 20.6kDa. Furthermore, the glucanohydrolase activities induced by UV-C were maintained until the end of the storage period. It is likely that the PR-proteins with glucanohydrolase activities induced by UV-C are an integral part of the long-term resistance observed in UV-C treated tomato fruit.
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