Pro-inflammatory effects and oxidative stress in lung macrophages and epithelial cells induced by ambient particulate matter

Michael, S.; Montag, M.; Dott, W.

Pro-inflammatory effects and oxidative stress in lung macrophages and epithelial cells induced by ambient particulate matter

2013

Michael, S. | Montag, M. | Dott, W.

The objective of this study was to compare the toxicological effects of different source-related ambient PM10 samples in regard to their chemical composition. In this context we investigated airborne PM from different sites in Aachen, Germany. For the toxicological investigation human alveolar epithelial cells (A549) and murine macrophages (RAW264.7) were exposed from 0 to 96 h to increasing PM concentrations (0–100 μg/ml) followed by analyses of cell viability, pro-inflammatory and oxidative stress responses. The chemical analysis of these particles indicated the presence of 21 elements, water-soluble ions and PAHs. The toxicological investigations of the PM10 samples demonstrated a concentration- and time-dependent decrease in cell viability and an increase in pro-inflammatory and oxidative stress markers.

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AGROVOC Keywords

al analysis animals as ca cat cd chemical analysis chemical composition chemical composition cr cu cytotoxicity elisa fe germany gsh humans humans ions macrophages macrophages mg mice mice mn na ni oxidation oxidative stress oxidative stress oxidative stress pah particulate matter particulate matter particulates pb polycyclic aromatic hydrocarbons polycyclic aromatic hydrocarbons rns sod tc toxicity zn

Bibliographic information

Published in

Environmental pollution

Volume 183 Pagination 19 - 29 ISSN 0269-7491

Publisher

Elsevier Ltd

Other Subjects

Raw264.7; Pm; Inflammatory response; Toxicological effects; Il; Tnf-α; Epithelial cells; Gpx; Pulmonary alveoli; Ufp; Stress response; Drug effects; Gssg; Voc; Sem; Grc; Lvs; A549; Ros; Air pollutants; Fbs; Alveolar; Nadp+/nadph; Cell viability; Cyp; Nf-kb; Pbs; Copd

Language

English

Type

Text; Journal Article

In AGRIS since: 2024-02-28

Format: MODS

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This bibliographic record has been provided by National Agricultural Library

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Links

DOI http://dx.doi.org/10.1016/j.envpol.2013.01.026

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