Determination of carotenoids and chlorophylls in water convolvulus (Ipomoea aquatica) by liquid chromatography
1992
Chen, B.H. | Chen, Y.Y.
The carotenoids and chlorophylls present in water convolvulus (Ipomoea aquatica) were analyzed by high-performance liquid chromatography (HPLC) with photodiode-array detection and their identify confirmed by thin-layer chromatography (TLC). An HPLC quaternary solvent system of acetonitrile methanol chloroform hexane (75:12.5:7.5:7.5, v/v/v/v) resolved 14 peaks in 20 min by using a 25 cm reversed-phase column, of which 12 pigments were identified. These pigments include carotenoids, chlorophylls and their isomers. The coefficient of variation for pigment concentrations was less than 12% in five sample analyses. A TLC quaternary solvent system of hexane acetone chloroform methanol in different proportions also resolved 10 pigments on silica gel layers. In addition to cis-lutein and cis-beta-carotene, all major pigments were adequately resolved by TLC.
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