Pathogenicity study of different avipoxviruses in embryonated chicken eggs and cell cultures
2007
Yādava, Śakuntalā | Dash, B.B. | Kataria, J.M. | Dhama, K. | Gupta, S.K. | Rahul, S.
Avipoxvirus infection, an economically important disease of domestic poultry and wild birds, is re-emerging in the recent past. Fowl poxvirus (FPV), quail poxvirus (QPV), turkey poxvirus (TPV) and pigeon poxvirus (PPV) could be adapted and propagated in chicken embryo fibroblast (CEF) cell culture, chorioallantoic membrane (CAM) of embryonated chicken eggs and QT-35 cell line with optimal titres. Studies were conducted on the pathogenicity of these viruses in these different host systems, which produced characteristic cytopathic effect (CPE) in CEF cell culture and QT-35 cell line viz. presence of intracytoplasmic inclusions in hypertrophied cells and plaque/syncytia formation and the lesions on CAM were distinct pocks, hyperplasia of ectoderm and intracytoplasmic inclusions.
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