Immunohistochemical demonstration of mycobacterial antigens in sheep experimentally infected with Mycobacterium avium subspecies paratuberculosis
2006
Kumar, A.A. | Tripathi, B.N. | Jolhe, D.K.
The objective of the present study was to compare diagnostic efficacy of indirect immunoperoxidase (IPT) and Ziehl Neelsen's (ZN) methods in histological confirmation of paratuberculosis. Fifteen lambs were orally infected with a caprine isolate of M.a.paratuberculosis. A group of 8 lambs were kept in the contact with the infected group and 8 lambs as uninfected control. The animals of the infected and incontact groups were classified as suspected (2), mild (9), moderate (3) and severe (3) infection categories on the basis of severity of histological lesions and positivity in other diagnostic tests. Demonstration of acid-fast bacilli and M.a.paratuberculosis antigen in tissues of 15 experimentally infected sheep. Two sheep showing no lesions were negative in both tests. Out of 2 suspected and 9 mild cases, only IPT detected 1 and 6 cases, respectively. In moderate and severe cases, both IPT and ZN methods were equally sensitive with former giving more positive signals than the latter. The overeall sensitivity of IPT (76.5%) was found to be far superior as compared with ZN method (35.3%). The results suggested the utility of IPT in the diagnostic confirmation of histological lesions without demonstrable AFB.
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