The Characterization of Carbohydrate Constituents from Different Soil Profiles
1963
Gupta, Umesh C. | Sowden, F. J. | Stobbe, P. C. (Peter C.)
Orthic Podzol, Podzol with Permafrost, Orthic Gray Wooded, Dark Brown Solodized Solonetz and Orthic Black profiles were studied for the carbohydrate constituents. The samples were hydrolyzed and the resulting sugars were determined using paper and column chromatography. The soil hydrolyzates were usually analyzed for galactose, glucose, mannose, arabinose, xylose, ribose, fucose and rhamnose. Largest amounts of sugars (mg. per g. of organic matter) were found in the top layers of Orthic Podzol and Orthic Gray Wooded profiles and the lowest amount in the Orthic Black profile. The total content of sugars decreased considerably with depth in Orthic Podzol, Orthic Gray Wooded and Orthic Black profiles but there was little change with depths in the Dark Brown Solodized Solonetz and in the Podzol with Permafrost. In general glucose was dominant in all horizons and it constituted 42 to 54% of the total sugars in the organic horizon of the podzolic soils. The other sugars decreased in the following order: galactose, mannose, arabinose, xylose, rhamnose and fucose-ribose. Four to seven high Rf sugars were found in selected samples analyzed; they made up 6 to 13% of the total sugars.
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