HPLC-ESI-MS method for C60 fullerene mitochondrial content quantification
2018
Grebinyk, Anna | Grebinyk, Sergii | Prylutska, Svitlana | Ritter, Uwe | Matyshevska, Olga | Dandekar, Thomas | Frohme, Marcus
The presented dataset describes the quantification of carbon nanoparticle C₆₀ fullerene accumulated in mitochondria of human leukemic cells treated with nanostructure. Firstly, the high performance liquid chromatography–electro spray ionization–mass spectrometry (HPLC-ESI-MS) method was developed for quantitative analysis of pristine C₆₀ fullerene. Then, human leukemic cells were incubated with C₆₀ fullerene, homogenized and subjected to the differential centrifugation to retrieve mitochondrial fraction. The C₆₀ fullerene content was quantified by HPLC-ESI-MS in extracts of cellular fractions.This data article refers to the research article “C₆₀ Fullerene Accumulation in Human Leukemic Cells and Perspectives of LED-mediated Photodynamic Therapy” by Grebinyk et al. [1].
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