The use of chemical and infra-red spectral analyses to verify the identification of two species of duckweed, Lemna minor L. and Lemna gibba L. grown under defined conditions
1992
Vaughan, D. | Russell, J.D. | Cheshire, M.V. | Fraser, A.R.
Flat forms of the fronds of Lemna gibba L., used widely in laboratory experiments, can be easily confused with Lemna minor. Chemical analyses of the two species, grown under defined laboratory conditions, showed clear distinctions between the two species in relation to total protein content and the content of individual monosaccharides. Infra-red spectral analysis confirmed the chemical data for protein content and also revealed differences in the types of proteins present in the two species. Chemical and infra-red spectral analyses, particularly the amide I:carbohydrate absorbance ratio, provide a rapid and reliable means of verifying between the two species, which are often difficult to identify with certainty from morphological differences.
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