Genetic transformation in the grain legume Cicer arietinum L. (chickpea)
1993
Fontana, G.S. | Santini, L. | Caretto, S. | Frugis, G. | Mariotti, D.
In the grain legume Cicer arietinum L. (chickpea), the seed-derived embryo axes deprived of the apical meristem were able to regenerate adventitious shoots on Murashige and Skoog (1962) medium supplemented with kinetin. This protocol was suitable for Agrobacterium-mediated gene transfer by the co-cultivation technique. Chickpea transgenic plants showed neomycin phosphotransferase II and beta-glucuronidase activities and the presence in their genome of integrated bacterial DNA.
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