Surface sieving coordinated IMAC material for purification of His-tagged proteins
2017
Li, Senwu | Yang, Kaiguang | Liu, Lukuan | Zhao, Baofeng | Chen, Yuanbo | Li, Xiao | Zhang, Lihua | Zhang, Yukui
Tailor-made materials for the purification of proteins with His-tag was designed through synergizing the selectivity of surface sieving and metal ion affinity. By excluding impurity proteins out of the surface polymer network, such materials could purify His-tagged proteins from the crude cell lysis with purity up to 90%, improved by 14% compared to that obtained by the commercial metal chelating affinity materials. This study might promote the His-tagged protein purification to a new level.
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