Entrapment of anaerobic thermophilic and hyperthermophilic marine micro‐organisms in a gellan/xanthan matrix
2016
Landreau, M. | Duthoit, F. | Claeys‐Bruno, M. | Vandenabeele‐Trambouze, O. | Aubry, T. | Godfroy, A. | Le Blay, G.
AIMS: The aims of this study were (i) to develop a protocol for the entrapment of anaerobic (hyper)thermophilic marine micro‐organisms; (ii) to test the use of the chosen polymers in a range of physical and chemical conditions and (iii) to validate the method with batch cultures. METHODS AND RESULTS: The best conditions for immobilization were obtained at 80°C with gellan and xanthan gums. After 5‐week incubation, beads showed a good resistance to all tested conditions except those simultaneously including high temperature (100°C), low NaCl (<0∙5 mol l⁻¹) and extreme pH (4/8). To confirm the method efficiency, batch cultures with immobilized Thermosipho sp. strain AT1272 and Thermococcus kodakarensis strain KOD1 showed an absence of detrimental effect on cell viability and a good growth within and outside the beads. CONCLUSION: This suggests that entrapment in a gellan–xanthan matrix could be employed for the culture of anaerobic (hyper)thermophilic marine micro‐organisms. SIGNIFICANCE AND IMPACT OF THE STUDY: (Hyper)thermophilic marine micro‐organisms possess a high biotechnological potential. Generally microbial cells are grown as free‐cell cultures. The use of immobilized cells may offer several advantages such as protection against phage attack, high cell biomass and better production rate of desired metabolites.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by National Agricultural Library
