Time-dependent alterations in gene expression of interleukin-8 in the bronchial epithelium of horses with recurrent airway obstruction

Ainsworth, D.M.; Wagner, B.; Franchini, M.; Grunig, G.; Erb, H.N.; Tan, J.Y.

Time-dependent alterations in gene expression of interleukin-8 in the bronchial epithelium of horses with recurrent airway obstruction

2006

Objective-To evaluate time-dependent alterations in gene expression of chemokines in bronchial epithelium of recurrent airway obstruction (RAO)-affected horses and whether alterations resulted from increases in gene expression of interleukin (IL)-17 in cells isolated from bronchoalveolar lavage fluid (BALF). Animals-8 RAO-susceptible horses and 9 control horses. Procedure-In 2 experiments, both groups of horses were evaluated after being maintained on pasture and after being stabled and fed dusty hay for 1, 14, 35, and 49 days (experiment 1) or 14 and 28 days (experiment 2). In experiment 1, gene expression of IL-8, chemokine (C-X-C motif) ligand 1 (CXCL1), granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF), and Toll-like receptor 4 (TLR4) in epithelium and IL-8, IL-17, and TLR4 in BALF cells was measured. In experiment 2, bronchial biopsy specimens were evaluated for IL-8 immunoreactivity. Results-In RAO-susceptible horses after 14 days of challenge exposure, there was a 3- and 10-fold increase in gene expression of IL-8 for epithelial and BALF cells and an increase in IL-8 immunoreactivity in epithelial cells. Challenge exposure failed to alter gene expression of CXCL1, GM-CSF, G-CSF, and TLR4 in epithelial cells of any horses at any time point. During challenge exposure, gene expression of BALF cell IL-17 was downregulated in control horses (day 1) and upregulated in RAO-affected horses (day 35). Conclusions and Clinical Relevance-Epithelial-derived IL-8 may promote airway neutrophilia, but the inciting stimulus is unlikely to be IL-17 because upregulation of this gene is subsequent to that of IL-8 in epithelial cells.

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AGROVOC Keywords

allergens animal feed animal feeding animals bronchi bronchi cytology epithelium gene expression gene expression regulation gene expression regulation genetics hay horses horses immune response immunology lavage receptors washing

Bibliographic information

Published in

American journal of veterinary research

Volume 67 Issue 4 Pagination 669 - 677 ISSN 0002-9645

Other Subjects

Granulocyte colony-stimulating factor; Respiratory mucosa; Horse diseases; Animal diseases (physiological); Temporal variation; Horse diseases; Disease susceptibility; Leukocyte count; Recurrence; Toll-like receptors; Bronchoalveolar lavage fluid; Interleukin-8; Respiratory tract diseases; Interleukin-17; Recurrent airway obstruction; Pulmonary alveoli; Airway obstruction; Veterinary; Interleukin-8; Lymphocyte count; Granulocyte-macrophage colony-stimulating factor

Language

English

Type

Text; Journal Article

In AGRIS since: 2024-02-28

Format: MODS

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Links

DOI http://dx.doi.org/10.2460/ajvr.67.4.669

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