Visualizing "green oil" in live algal cells

Cooper, Mark Scott; Hardin, William Robert; Petersen, Timothy Wayne; Cattolico, Rose Ann

Visualizing "green oil" in live algal cells

2010

Cooper, Mark Scott | Hardin, William Robert | Petersen, Timothy Wayne | Cattolico, Rose Ann

We report here that BODIPY 505/515, a green lipophilic fluorescent dye, serves as an excellent vital stain for the oil-containing lipid bodies of live algal cells. BODIPY 505/515 vital staining can be used in combination with fluorescent activated cell sorting to detect and isolate algal cells possessing high lipid content.

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AGROVOC Keywords

boron compounds chemistry cytology eukaryota flow cytometry lipid content microalgae microscopy

Bibliographic information

Published in

Journal of bioscience and bioengineering

Volume 109 Issue 2 Pagination 198 - 201 ISSN 1389-1723

Publisher

Society for Bioscience and Bioengineering, Japan; Amsterdam, the Netherlands

Other Subjects

Mallomonas splendens; Lipid bodies; Bodipy 505/515; Plant physiology and biochemistry; Fluorescent dyes; Aquatic sciences related; Ophiocytium maius; Fluorescent dyes; Chrysochromulina; Confocal

Language

English

Note

Includes references

Type

Journal Article; Text

In AGRIS since: 2024-02-28

Format: MODS

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Links

DOI DOI http://dx.doi.org/10.1016/j.jbiosc.2009.08.004

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Visualizing "green oil" in live algal cells

2010

AGROVOC Keywords

Bibliographic information