Sulfation of Agrocybe chaxingu polysaccharides can enhance the immune response in broiler chicks
2013
Zhang, Xuhui | Cao, Fuliang | Sun, Zhiyuan | Yu, Wanwen | Zhao, Linguo | Wang, Tian
In this study, our objective was to compare the effects of sulfated Agrocybe chaxingu polysaccharide (sACP) and nonsulfated ACP (nACP) on lymphocyte proliferation in vitro and immune-enhancing activity in broiler chicks. In the in vitro test, 3 sACP with different degrees of sulfation—sACP₀.₈₃, sACP₁.₃₂, and sACP₁.₇₉—were used to study the peripheral lymphocyte proliferation using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay with nACP as the control. On the basis of this test, sACP₁.₃₂ and sACP₁.₇₉ were selected for the in vivo test. In the in vivo test, 300 one-day-old chicks were randomly divided into 5 groups (negative control, vaccine control, nACP, sACP₁.₃₂, and sACP₁.₇₉). Chicks in the vaccine control and ACP groups were vaccinated with Newcastle disease (ND) vaccine at 10 d of age, with a booster dose at 21 d of age. At the time of the first vaccination, the chicks in the 3 ACP groups were injected with nACP, sACP₁.₃₂, and sACP₁.₇₉, whereas those in the vaccine control group were injected with 0.5 mL of physiological saline once a day for 3 successive days. In comparison to nACP, sACP₁.₃₂ and sACP₁.₇₉ significantly increased the relative weight of the thymus and spleen at 22 d of age, enhanced serum ND antibody titers at 16, 22, 28, 34, and 42 d of age, and enhanced splenic T- and B-lymphocyte proliferation at 22 and 28 d of age. Furthermore, sACP₁.₃₂ and sACP₁.₇₉ could induce significant augmented levels of interferon-γ and IL-6. Sulfation could enhance both cellular and humoral immunity in broiler chicks on a degrees of sulfation basis. The sACP₁.₇₉ exerted the best efficacy and would be expected to be immunopotentiator molecule during ND vaccination in broilers.
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