Improved HPLC determination of hydroxycinnamic acids in orange juice using solvents containing THF
1992
Rouseff, R.L. | Seetharaman, K. | Naim, M. | Nagy, S. | Zehavi, U.
Solvent optimization techniques were used to maximize chromatographic resolution between sinapic, coumaric, ferulic, and caffeic acids and components in orange juice. Various reversed-phase solvent systems consisting of binary, ternary, and quaternary combinations of methanol, acetonitrile, and THF with water were evaluated. Greatest chromatographic resolution was realized from binary mobile phases consisting of THF and water and ternary solvents containing THF, methanol, and water. Resolution between the least resolved peak pair was improved 46% using the binary THF mobile phase compared to the best previously reported mobile phase. The THF, methanol, and water mobile phase produced the greatest resolution between the four acids of interest; however, unknown components in orange juice coeluted with both the sinapic and caffeic acid peaks. The mobile phase consisting of 21% THF/79% water (with 2% acetic acid) produced the best separation and allowed the most accurate quantitation of the four hydroxycinnamic acids from the components in orange juice. Chromatographic behavior may differ if acids other than acetic are used to suppress ionization.
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