Malaria vaccine antigen(s): Detergent solubilization, partial isolation, and recovery of immunoprotective activity
1984
D'Antonio, L.E. | Keshavarz-Valian, H. | Alger, N.E.
Plasmodium berghei-infected erythrocytes were solubilized with nonionic detergent n-octyl glucoside and membrane-containing P. berghei fragments with either n-octyl glucoside or ionic detergent sodium deoxycholate, unsolubilized material was separated by centrifugation and detergent was removed from respective supernatants by gel filtration, reaggregated components in respective void volume eluates acted as specific vaccine in recipient mice
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