Characterisation of four Leuconostoc bacteriophages isolated from dairy fermentations
1995
Davey, G.P. | Ward, L.J.H. | Brown, J.C.S.
Four bacteriophages (phages) growing on the same Leuconostoc strain were characterized. Electron micrographs showed these phages to he similar in morphology to the commonly isolated lactococcal phages with head diameters ranging from 49-55 nm and tail lengths of 117-131 nm. A distinctive base plate and collar were also present. From restriction enzyme analysis of purified phage DNA, the genome sizes were 23-29. All four phages showed one major structural protein (of approximately 74 kDa) on SDS polyacrylamide gels. Hybridization experiments confirmed that the phages belonged to the same homology group. There was no homology between DNA from these phages and DNA from a prolate or small isometric lactococcal phage.
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