Effect of growth hormone transgenic Synechocystis on growth, feed efficiency, muscle composition, haematology and histology of turbot (Scophthalmus maximus L.)
2007
Liu, Shunmei | Zang, Xiaonan | Liu, Bin | Zhang, Xuecheng | K.K.I U., Arunakumara | Zhang, Xiaoqing | Liang, Bing
The present study investigated the effect of supplementing feed with transgenic Synechocystis sp. PCC6803 containing the Paralichthys olivaceus growth hormone (GH) gene on growth, feed intake and feed efficiency ratio, muscle composition, haematology and histology of turbot (Scophthalmus maximus L.). At the end of the 40-day feeding trial, the specific growth rate of fish fed the supplemented feed with 1.0% transgenic Synechocystis sp. PCC6803 was 21.67% higher (P<0.05) than that of control fish. Although body weight and feed efficiency ratio significantly increased (P<0.05) in fish fed the diet supplemented with transgenic alga, feed intake and condition factor of the experimental fish were unaffected. Muscle composition analysis showed that the protein content was positively influenced by the transgenic alga, whereas the lipid content was unaffected. Haematological parameters, including red blood cell, white blood cell, haemoglobin, and serum biochemical indices, such as enzyme activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase, concentrations of total protein, glucose, blood urea nitrogen, creatinine, triglyceride and cholesterol and ion levels of K, Na, Cl, P were not influenced by supplementing the transgenic Synechocystis sp. PCC6803. Furthermore, no histopathological alterations were induced by transgenic alga treatment in the stomach, intestine, liver, spleen and kidney of the experimental fish. The results of the present study indicated that transgenic Synechocystis sp. PCC6803 containing P. olivaceus GH gene is an efficient growth promoter and a safe feed additive for fish.
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