Local antitumor effects of intratumoral delivery of rlL-2 loaded sustained-release dextran/PLGA–PLA core/shell microspheres
2013
Zhao, Haiping | Wu, Fei | Cai, Yunpeng | Chen, Yinghui | Wei, Liangming | Liu, Zhenguo | Yuan, Weien
In this study, we formulated a rIL-2 loaded sustained-release dextran/PLGA–PLA core/shell microsphere, mimicking the paracrine mechanisms of cytokine action, to investigate its local antitumor efficacy. The presented microspheres were formed in two steps: rIL-2 was firstly loaded into dextran particles to keep its bioactivity by a unique method of stabilizing aqueous–aqueous “emulsion”; subsequently, the particles were encapsulated into poly(dl-lactide-co-glycolide)/polylactic acid (PLGA/PLA). A stable sustained release behavior in vitro was achieved for a period of about 25 days. In the subcutaneous colon carcinoma BALB/c mice models, a single dose of microspheres was introtumorally administrated and compared with multiple doses of rIL-2 solution to investigate the long acting effect of microspheres on tumor. The animal experiments showed the local efficacy at tumor site mediated by rIL-2 from a single dose of microspheres was better than that of multiple rIL-2 solution injections. Based on the experimental results, we conclude that rlL-2 loaded sustained-release dextran/PLGA–PLA core/shell microspheres represent a promising approach for local cancer treatment in animals.
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