Effect of culture origin and conditions on duvatrienediol accumulation in shoot cultures of tobacco
1992
Miedzybrodzka, M.B. | Yeoman, M.M.
A comparison of morphology, trichome type and duvane accumulation of tobacco shoot cultures originating from seedlings, callus and cell suspension cultures incubated on a range of cytokinin concentrations is presented. A method for the extraction and HPLC analysis of duvatrienediols from cultures is described. Duvatrienediols (DVTs) were not detected in callus or cell suspension cultures and were only detected in seedling-derived shoot cultures. Shoot development was dependent on explant type probably as a result of pre-exposure of callus and suspension cells to high auxin concentrations. Low cytokinin levels promoted shoot development whilst high cytokinin levels increased the number of shoots regenerated but retarded their development. DVT accumulation occurred only in 'normally' developed shoots with trichomes. Growth of shoot cultures in liquid medium was inhibitory to the accumulation of DVTs. Optimum conditions for the accumulation of DVTs were shown to be incubation of seedling-derived shoot cultures on media containing 10(6)M BAP solidified with agar. The yield was 120 micrograms g-1 fr. wt. which is comparable to greenhouse-grown material. Both alpha and beta-DVT were detected in a ratio of 5:1. The relationship between trichomes and DVT accumulation is discussed with reference to the roles of culture origin, cytokinin, trichome type and environmental effects.
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