Characterization and properties of the biosurfactant produced by Candida lipolytica UCP 0988
2014
Rufino, Raquel Diniz | de Luna, Juliana Moura | de Campos Takaki, Galba Maria | Sarubbo, Leonie Asfora
Biotechnological processes are costly, especially for the production of biosurfactants. The successful production of a biosurfactant is dependent on the development of processes using low cost raw materials. Considering the importance of the characteristics of a biosurfactant to facilitate its industrial application, the properties of the biosurfactant produced by Candida lipolytica through previously optimized medium have been established.The yeast was grown for 72h to determine the kinetics of growth and production. The surface tension of the cell-free broth was reduced from 55 to 25mN/m. The yield of biosurfactant was 8.0g/l with a CMC of 0.03%. The biosurfactant was characterized as an anionic lipopeptide composed of 50% protein, 20% lipids, and 8% of carbohydrates.The isolated biosurfactant showed no toxicity against different vegetable seeds: Brassica oleracea, Solanum gilo and Lactuca sativa L. and the micro-crustacean Artemia salina. The properties of the biosurfactant produced suggest its potential application in industries that require the use of effective compounds at low cost.
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