Supercritical carbon dioxide extraction of essential oils from Perovskia atriplicifolia Benth
2003
Pourmortazavi, S.M. | Sefidkon, F. | Hosseini, S.G.
The supercritical fluid extraction (SFE) of the aerial parts of Perovskia atriplicifolia Benth. was studied. The effect of different parameters such as pressure, temperature, modifier identity, and modifier volume on the SFE of the plant was investigated. The extracts were analyzed by GC and GC-MS and compared with the essential oil obtained from P. atriplicifolia Benth by steam distillation. The supercritical extracts and the steam-distilled products had very different compositions. The main constituents of the oil obtained by steam distillation were 1,8-cineole, limonene, camphor, β-caryophyllene, α-pinene, camphene, and α-humulene. On the other hand, the major components of SFE extracts were 1,8-cineole, limonene, camphor, β-caryophyllene, γ-cadinene, α-pinene, and α-terpinyl acetate. The results showed that increasing the temperature from 35 to 65 °C (at a constant pressure of 100 atm) drastically reduced the number of extracted components. Also, the number of extracted constituents and the percent of main analytes increased when lower pressures were used. Using different modifiers (e.g., methanol, ethanol, dichloromethane, and hexane) for the extraction of the plant at low pressure (100 atm) and temperature (35 °C) showed that hexane was more selective than the other modifiers.
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