Identification of genes regulated by low temperature in Pachysandra terminalis Sieb. et Zucc using cDNA differential display
2005
Zhou, S. | Sauve, R.J. | Abdulah, A.
Complementary Deoxyribonucleic Acid (cDNA) differential display and reverse Northern dot blot were used to identify genes in Pachysandra terminalis Sieb. & Zucc., a cold-tolerant plant, that are regulated by low temperatures. Rooted cuttings were obtained from stock plants that had been maintained in a greenhouse at 24 degrees C. These cuttings were subjected to the following cold treatments: 2 weeks at 12 degrees C, 48 hours at 4 degrees C, 48 hours at 0 degrees C, and 4 hours at -1 degrees C. Following leaf tissue analysis of treated and control plants, some stress-related genes and many novel genes were identified. Northern blot hybridization demonstrated that all novel genes were regulated by the cold treatments.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by National Agricultural Library