p66Shc-mediated hydrogen peroxide production impairs nephrogenesis causing reduction of number of glomeruli
2021
Miller, Bradley | Palygin, Oleg | El-Meanawy, Ashraf | Mattson, David L. | Geurts, Aron M. | Staruschenko, Alexander | Sorokin, Andrey
Adaptor protein p66Shc, encoded by Shc1 gene, contributes to the pathogenesis of oxidative stress-related diseases. p66Shc ability to promote oxidative stress-related diseases requires phosphorylation of serine 36 residue (Ser36) and depends on translocation of p66Shc to the mitochondria. We tested the hypothesis that abnormal p66Shc-mediated reactive oxygen species (ROS) production could be critically involved in nephrons development during nephrogenesis. We have generated unique mutant rats (termed p66Shc-Del), which express endogenous p66Shc with a 9-amino acid deletion, and lack regulatory Ser36. H₂O₂ renal production was measured by enzymatic microelectrode biosensors. Nephron numbers in 3–5 weeks old p66Shc-Del rats were quantified using the acid maceration method. p66Shc-Del rats, as wild type salt sensitive rats, display increased mean arterial blood pressure following chronic exposure to a high salt diet. In contrast to wild type rats, p66Shc-Del rats display increased H₂O₂ renal production and are characterized by a reduction in renal function. The number of glomeruli is significantly reduced in adult p66Shc-Del rats. Since low nephron number is an established risk factor for kidney disease and hypertension in humans and rodents, our data suggest that H₂O₂ renal production, caused by irregular signaling of p66Shc, could be critical in regulating nephrogenesis and that abnormal p66Shc signaling negatively impacts kidney development and renal function by increasing susceptibility to diabetic nephropathy and hypertension-induced nephropathy.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by National Agricultural Library