Bacteriocin partitioning from a clarified fermentation broth of Lactobacillus plantarum ST16Pa in aqueous two-phase systems with sodium sulfate and choline-based salts as additives
2018
Sabo, Sabrina da Silva | Lopes, André Moreni | Santos-Ebinuma, Valéria de Carvalho | Rangel-Yagui, Carlota de Oliveira | Oliveira, Ricardo Pinheiro de Souza
The partitioning of bacteriocin, a promising alternative to chemical preservatives, in a novel inexpensive and stable aqueous two-phase system (ATPS) comprising poly(ethylene glycol) (PEG) and sodium polyacrylate (NaPA) was studied. The ATPS was generated by mixing both polymers with Na2SO4 or choline chloride ([Ch]Cl) and a bacteriocin extract from the fermented broth of Lactobacillus plantarum ST16Pa. Bacteriocin showed stability at different pH values (3.0–8.0) and temperatures (50–80°C), as well as in the presence of ATPS components. Hydrophobic and electrostatic interactions were found to be the major driving forces for bacteriocin partitioning. The peptide partitioned preferentially to the PEG-rich phase (partition coefficient, KBact>1). However, the highest partition coefficient was achieved in the polymeric-based ATPS using [Ch]Cl as additive, as follows: 8wt% PEG 10,000g/mol/8wt% NaPA 8,000g/mol/0.5M [Ch]Cl, resulting in a KBact equal to 32. Moreover, these conditions promoted high selectivity (S=62.7), since the greater part of total proteins partitioned into the NaPA-rich phase (Kprot=0.51). In conclusion, ATPS composed of PEG/NaPA using choline-based salts as additive can be considered mainly as a first step for bacteriocin extraction from fermented broth by applying low polymer content and mild conditions.
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