In vitro propagation of Anthyllis barba-jovis from seedling tissues
2017
Trigka, M. | Papafotiou, M.
Anthyllis barba-jovis L. (f. Fabaceae, Jupiter's Beard) is a native, silvery evergreen shrub, bearing big inflorescences with pale yellow flowers from May to June, found in rocky cliffs of Mediterranean region. Being very tolerant to high salinity, wind and drought, it could be introduced as an ornamental landscape plant for coastal Mediterranean areas. Micropropagation of the species was studied using explants from young seedlings. Seeds germinated on half-strength Murashige and Skoog (MS) medium at 15°C. Cotyledonary nodes from 33-day-old seedlings were used as explants and cultured on hormone-free (Hf) MS medium or MS medium supplemented with either 0.5 or 1.0 mg L-1 benzyladenine (BA) alone or in combination with 0.1 mg L-1 naphthaleneacetic acid (NAA), or with 0.1 mg L-1 NAA alone. In all treatments, almost all explants provided shoots (92-98%). Higher shoot production was promoted by 1.0 mg L-1 BA with or without NAA (1.9 shoots per explant). A subculture took place on Hf-MS medium or MS medium supplemented with BA (0.5 or 1.0 mg L-1) combined with 0.1 mg L-1 NAA. Shooting reached 91-98% in all treatments. Higher shoot production was promoted by 1.0/0.1 mg L-1 BA/NAA (8.1 shoots per explant), while shoot length was similar in all media (1.2-1.3 cm). At BA-media, particularly at the higher BA concentration, about half of the explants apart from the normal shoots reported above, bear hyperhydric shoots (about 4). Microshoots rooted at 90% on half-strength Hf MS medium and at 100% supplemented with 0.5 mg L-1 indole-3-butyric acid (IBA), while 2 mg L-1 IBA induced 98% rooting and the greatest number of roots (4.3). Ex vitro acclimatization was highly successful (97%) on a mix of 1 peat:1 perlite (v/v).
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