Insights about the toxicity of tannery effluent on chicken (Gallus gallus domesticus) embryos
2020
Gomes, Alex Rodrigues | Andrade Vieira, Julya Emmanuela de | Costa Araújo, Amanda Pereira da | Malafaia, Guilherme
Although tannery effluent (TE) toxicity has already been demonstrated in different vertebrate models, our knowledge about their effects on birds remains significantly incipient. Thus, the aim of the current study was to evaluate the impact of ephemeral exposure of Gallus gallus domesticus eggs to environmental predictive TE dilutions (1.4% and 6.5%). Eggs at E6 developmental stage were opened in order to assess embryos’ external morphology and genotoxic biomarkers. Based on our data, embryos exposed in ovo to TE recorded higher mortality rate, lower biomass and different morphological abnormalities such as optic vesicle depigmentation, pericardial and encephalic edemas, as well as body rotation error. Embryos exposed to TE showed lower crown-rump length head and anterior-posterior length, as well as reduced beak size. Embryos exposed to the highest TE dilution (6.5%) also showed greater lower/upper limb development, larger optic vesicle area and smaller crystalline lens area than the other groups. On the other hand, differences in mitotic index were not observed between groups; however, total erythrocyte chromosomal abnormalities, mainly in metaphase and anaphase, were higher in embryos exposed to TE. These phases presented chromosome fragments formed from typical chromosome breakage, laggard chromosome and chromosome bridge. Higher Cr, Mn and Zn concentrations in embryos exposed to TE strongly suggest that the observed abnormalities were directly associated with the absorption of chemical constituents. The present study is pioneer in investigating the morphotoxic and genotoxic potential of TE (a complex mixture of various xenobiotics) in bird embryos in order to better understand the eco (toxicological) magnitude of this pollutant in aquatic ecosystems.
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