Transgenic sequences detected in corn, soybean and cotton grains imported to Mexico
2012
Macfas-de la Cerda, Claudia Gabriela | Cantú-lris, Mariana | Cruz-Requena, Marisol | Rodrfguez-Herrera, Raúl | González-Vázquez, Victor Manuel | Aguilar, Cristóbal Noé | Loyola-Licea, Juan Carlos | Contreras-Esquivel, Juan Carlos
Transgenic grain of different crops is imported to Mexico without any reference. In this study, 29 samples of corn, 22 of soybean and 21 of cotton from different lots were analyzed by Polymerase Chain Reaction (PCR). PCR wascarried out using specific primers for accompanying sequences (ubi, 3SS, npt II, bar, gus, luc, us, ocs) and 9 transgenic events (crylab, cryle, cry2A, cry3A, cry11A, epsps, als, ec, accasa). Results showed that all the analyzed samples contained at least one transgenic sequence. The most frequently detected promoter in the imported grains was ubi (83%). The marker most detected in the samples was ntpll (75%), while the reporter most detected was luc (47%) and the terminator ocs in 65%. On the other hand, the most detected transgenic event was epsps (82%) of the cases. The results suggest that the great majority of corn, soybean and cotton grain lots that Mexico imports are mixtures of genetically modified varieties.
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