Salinity stress and hydrogen peroxide regulation of antioxidant defense system in Ulva fasciata
2006
Lu, Yifan | Sung, Ming-Shiuan | Lee, Tse-Min
The regulation of antioxidant defense system in macroalgae exposed to salinity stress was examined in Ulva fasciata Delile. As compared to the 30‰ control, a long-term (4 days) exposure to hyposaline (5, 15‰) and hypersaline (60, 90, 120, 150‰) conditions inhibited growth rate and TTC reduction ability. A decrease in maximum quantum efficiency (F ᵥ/F ₘ ratio) and the maintenance of superoxide dismutase activity under salinity stress indicate the potential generation of reactive oxygen species in chloroplasts. An exposure to 15, 60, and 90‰ decreased seawater H₂O₂ contents but increased thallus H₂O₂ contents that are positively correlated with TBARS and peroxide contents. Alleviation of oxidative damage and H₂O₂ accumulation at 15 and 90‰ by a H₂O₂ scavenger, dimethylthiourea, suggests that oxidative damage occurring under moderate hyposaline and hypersaline conditions is ascribed to accumulated H₂O₂. Increased glutathione reductase activity and glutathione content and decreased ascorbate content are responsible for accumulated H₂O₂ at 15, 60, and 90‰, while ascorbate peroxidase activity increased only at salinity ≥ 90‰. Catalase and peroxidase activities also increased at 60 and 90‰ for H₂O₂ removal, but only catalase showed activity increase at 15‰. For the regeneration of ascorbate, the activities of both dehydroascorbate reductase and monodehydroascorbate reductase were increased at 5 and 15‰ while only monodehydroascorbate reductase activity increased at 60 and 90‰. It is hypothesized that the availability of antioxidants and the activities of antioxidant enzymes are increased in U. fasciata to cope with the oxidative stress occurring in hyposaline and hypersaline conditions.
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