Isolation, purification and characterization of neutral polysaccharides from extracted apple juices
1994
Will, F. | Dietrich, H.
Using membrane processes, the pectic material of an extracted apple juice was isolated, decolorized and degraded with purified pectin esterase (EC 3.1.1.11) and endo-polygalacturonase (EC 3.2.1.15). Separation of the resulting polysaccharides by anion-exchange-chromatography yielded two neutral and one acidic fractions. Enzymic hydrolysis with purified arabinofuranosidase, endo-alpha-1,5-arabanase and partial acid hydrolysis in combination with methylation analysis revealed linear alpha-L-1,5-arabans, highly branched type II-arabinogalactans and rhamnogalacturonans with a high content of neutral sugars, which could not be further degraded by purified pectinases. The isolated polymers are involved in clarification and stabilization problems of apple juice or concentrate.
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