Effects of gamma irradiation on edible seed protein, amino acids and genomic DNA during sterilization
2009
Maity, Jyoti Prakash | Chakraborty, Sukalyan | Kar, Sandeep | Panja, Subrata | Jean, Jiin-Shuh | Samal, Alok Chandra | Chakraborty, Anindita | Santra, Subhash Chandra
This work describes radiation-induced effects on edible seed protein profiles, carbohydrates, amino acids and genomic DNA during gamma sterilization. The total protein and carbohydrate was decreased with increasing dose compared to control samples. Oryza sativa L. Cv-2233 exhibited a minimum effect in terms of its loss in total soluble protein content, compared to other seeds at 6kGy, and the soluble protein fraction, containing 14-16kDa albumins and 22kDa globulin, was unchanged up to 6 kGy. In Cicer arietinum, the effect of gamma rays was more pronounced on albumin and prolamin with respect to glutelin and globulin. The easy-to-digest and difficult-to-digest proteins were not significantly affected up to 4kGy. However, the soluble free amino acids of all the seeds increased with increasing dose. The total DNA content and band intensities both decreased with increasing absorbed dose; however, the band positions were unchanged for all seed types.
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