Rapid bacteria identification from environmental mining samples using MALDI-TOF MS analysis
2017
Avanzi, Ingrid Regina | Gracioso, Louise Hase | Baltazar, Marcela dos Passos Galluzzi | Karolski, Bruno | Perpetuo, Elen Aquino | Nascimento, Cláudio Augusto Oller do
Copper mining has polluted soils and water, causing a reduction of the microbial diversity and a change in the structure of the resident bacterial communities. In this work, selective isolation combined with MALDI-TOF MS and the 16S rDNA method were used for characterizing cultivable bacterial communities from copper mining samples. The results revealed that MALDI-TOF MS analysis can be considered a reliable and fast tool for identifying copper-resistant bacteria from environmental samples at the genera level. Even though some results were ambiguous, accuracy can be improved by enhancing reference databases. Therefore, mass spectra analysis provides a reliable method to facilitate monitoring of the microbiota from copper-polluted sites. The understanding of the microbial community diversity in copper-contaminated sites can be helpful to understand the impact of the metal on the microbiome and to design bioremediation processes.
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