Production of a novel raw-starch-digesting glucoamylase by Penicillium sp. X-1 under solid state fermentation and its use in direct hydrolysis of raw starch
2007
Sun, Haiyan | Ge, Xiangyang | Zhang, Weiguo
Penicillium sp. X-1, isolated from decayed raw corn, produced high level of raw-starch-digesting glucoamylase (RSDG) under solid state fermentation (SSF). Maximum enzyme yield of 306.2 U g-¹ dry mouldy bran (DMB) was obtained after 36 h of culture upon optimized production. The enzyme could hydrolyse both small and large granule starches but did not adsorb on raw starch. The enzyme exhibited maximum activity at 65°C and pH 6.5, which provided an opportunity of synergism with α-amylase. It significantly hydrolysed 15% (w/v) raw corn starch slurry in synergism with the commercial α-amylase and a degree of hydrolysis of 92.4% was obtained after 2 h of incubation.
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