Evaluation of total protein content in tears of dogs by polyacrylamide gel disk electrophoresis

Barrera, R.; Jimenez, A.; Lopez, R.; Mane, M.C.; Rodriguez, J.F.; Molleda, J.M.

Evaluation of total protein content in tears of dogs by polyacrylamide gel disk electrophoresis

1992

Concentration of total proteins was measured and sodium dodecyl sulfate-polyacrylamide gel disk electrophoresis was performed on tear and plasma samples obtained from 26 healthy dogs, and the results were compared. Mean +/- SEM concentration of total proteins in tears was 0.63 +/- 0.04 g/dl, and significant effects of age or gender were not found. The protein composition of tears in dogs was complex, and bands from light and heavy chains of immunoglobulins were identified by electrophoresis.

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AGROVOC Keywords

age differences analysis animals blood plasma blood proteins chemistry densitometry dogs dogs electrophoresis electrophoresis metabolism protein content tears

Bibliographic information

Published in

American journal of veterinary research

Volume 53 Issue 4 Pagination 454 - 456 ISSN 0002-9645

Other Subjects

Protein composition; Gender differences; Female; Disc; Eye proteins; Male; Reference values; Immunoglobulin light chains; Age factors; Immunoglobulin heavy chains

Language

English

Note

2019-12-04

Type

Text; Journal Article

In AGRIS since: 2024-02-28

Format: MODS

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